NMR

EPP has it’s own in-house, GLP compliant, NMR facility supported by a dedicated and highly experienced NMR operator with many years’ experience in the field. As a result we can offer a stand alone NMR service to clients in addition to supporting our other EPP services.

Our NMR spectrometer is a Bruker Avance 500MHz running Topspin and IconNMR. Samples can be run automatically with our 60 position sample changer. We can offer NMR experiments for 1H, 13C, 31P and other relevant nuclei (excluding 19F). We have experimental methods in place for 1D, 2D and Quantitative NMR. The instrument is fitted with variable temperature control, pulsed field gradients and shaped pulses.

An EPP chemist works in the NMR laboratory

Operating the NMR – Bruker Avance 500MHz.

We can identify compounds using 1D spectra from 1H, 13C and other nuclei, and various 2D experiments, including COSY, HSQC, NOESY and HMBC.  Experiments using other 2D methods and other nuclei can be set up if required.

We can undertake quantitative NMR experiments to characterise materials and to certify standards examples being Ethephon, Glyphosate and Glufosinate.

 

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EPP is a member of SNUG, an alliance of Scottish NMR groups from the universities of Aberdeen, Edinburgh, Dundee, Heriot-Watt, Glasgow, St Andrews, Strathclyde, the Beatson Institute for Cancer Research and EPP Ltd.

How long do these services take?

For 1D Experiments without interpretation and sample weight >10mg, we can achieve a 24 hour turnaround.

If you are interested in finding out more about our NMR services please contact Lorna Kennedy.

Our NMR services include:

  • NMR stand alone service (GLP)
  • NMR certified reference standards
  • Structural elucidation

NMR stand alone service (GLP)

Our stand alone NMR service is fully GLP compliant and we can offer a rapid turnaround with spectral assignments if required. We have the capacity to offer long acquisition periods in order to extract NMR data from weak samples

NMR certified reference standards

Using Quantitative NMR (with use of an internal standard) we have established “Gold Standards” of purity and certification of active ingredients, impurity and metabolite analytical reference standards. This approach can be far superior to other analytical methods. For example, purity assessment by HPLC-UV where different components such as impurities can have different response factors to the UV wavelength used, potentially leading to an inaccurate assignment of purity.

Structural elucidation

Essential for understanding the outcomes of synthetic reactions and the identification of impurities in technical materials, the structural elucidation of chemical entities plays a key part across our services. We have state-of-the-art instruments and facilities, operated by specialists, to ensure this process is as accurate and efficient as possible. We report against GLP compliance and can supply an example report on request.